Tehran University of Medical Sciences Journal of Medical Ethics and History of Medicine 2008-0387 18 0 2025 10 18 1580 1580 Alireza Khalili Golestan University of Medical Sciences, Gorgan, Iran. Saeedeh Saeedi Tehrani Assistant Professor, Department of Medical Ethics, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. Mahshad Noroozi Assistant Professor, Department of Medical Ethics, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. Soodabeh Hoveidamanesh Assistant Professor, Preventive Medicine and Public Health Research Center, Psychosocial Health Research Institute, Department of Community and Family Medicine, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. Arezou Baradaran Education Development Office, Rasool Akram Medical Complex, Iran University of Medical Sciences, Tehran, Iran. Akram Hashemi Assistant Professor, Center for Educational Research in Medical Science (CERMS), Department of Medical Education, School of Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran; Department of Medical Ethics, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. 2025 07 12 2025 10 18 Professionalism impacts professional identity, staff efficiency, and patient care quality. The purpose of this study was to evaluate the professional behavior and climate in a teaching hospital in Iran. A cross-sectional study was performed among faculty members, nursing staff, and specialized residents. The study used the validated 30-item "Medical Professionalism Climate in Clinical Settings" questionnaire developed by Asghari et al., containing two domains: adherence to professional behavior, and perceptions of the professional climate. Data were analyzed using SPSS version 26. The study population consisted of 271 individuals working at a teaching hospital, 44.3% male and 55.7% female, averaging 34.79 years in age.The results showed there was a relationship between the total mean score, the professional behavior score, the professional climate score, and the participants' professional titles (P-value < 0.001), as well as the duration of their work experience in the department (P-value: 0.005, P-value: 0.039, P-value: 0.001, respectively). The faculty members achieved the best scores, followed by the residents, nurses, and nursing assistants. Analysis of the professional behavior scores revealed strengths and weaknesses among the medical staff. More training is recommended to enhance skills and promote positive behavior to boost hospital staff's job satisfaction and sense of belonging. https://jmehm.tums.ac.ir/index.php/jmehm/article/view/1580 https://jmehm.tums.ac.ir/index.php/jmehm/article/download/1580/475